repressing of sox6 and sox9 in situ chondrogenic differentiation of rat bone marrow stromal cells

نویسندگان

mohammad hassan karimfar department of anatomical sciences, faculty of medicine, zahedan university of medical sciences, zahedan, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (zahedan university of medical sciences)

aliasghar peyvandi hearing disorders research center, shahid beheshti university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences)

mohsen noorozian department of biology and anatomical sciences, school of medicine, shahid beheshti university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences)

navid ahmadi roozbahani hearing disorders research center, shahid beheshti university of medical sciences, tehran, iran.سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences)

چکیده

introduction: sox9 is a transcriptional activator which is necessary for chondrogenesis. sox6 are closely related to dna-binding proteins that critically enhance its function. therefore, to carry out the growth plate chondrocyte differentiation program, sox9 and sox6 collaborate genomewide. chondrocyte differentiation is also known to be promoted by glucocorticoids through unknown molecular mechanisms. methods: we investigated the effects of asynthetic glucocorticoid, dexamethasone (dex), on sox9 gene expression in chondrocytes. results: sox9 mrna was expressed at high levels in these chondrocytes. treatment with dex resulted in enhancement of sox9 mrna expression. the dex effect was dose dependent (0·5 nm and 1 nm). conclusion: rt-pcr analysis revealed that dex also enhanced the levels of sox9 expression. it was observed that dex had enhancing effect only on sox9 the expression level was low for sox6. it can thus be concluded that chondrocyte differentiation can be promoted by dex via sox9 enhancement.

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anatomical sciences journal

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